tools - under construction
Hahn
constructs
at Addgene
Hahn lab DNA constructs are available at Addgene. Addgene is a nonprofit that produces the constructs with good quality control and simplifies paperwork.
LINK: Spreadsheet of molecular imaging tools - This Excel spreadsheet lists our molecular imaging tools (e.g. biosensors, optogenetics, chemogenetics). You can sort by target molecule, design, purpose etc. Each design has its own advantages and caveats. The designs designated in the spreadsheet are explained in the ‘Designs’ section below.
Overview of our optogenetics tools as of 2020: Liu, B., Marston, D.J., and Hahn, K.M. Engineering optogenetic protein analogs. Meth. Mol. Biol., 2173: 113-126, 2020. PMC7448381 Online article | Free PMC article
Please let us know if we can help, or if you encounter problems with the tools. We would appreciate feedback.
Designs (under construction)
- antenna
- dual chain AR
- dual chain AR / dye
- dye analog
- dye-AR / endogenous
- embedded pep
- embedded pep/Zdk
- iLID
- insertion analog
- loopology
- LOVTRAP
- PA
- RapR
- RapR TAP
- single chain AR
- sm mol
- SNAPSense
- SPELL Split
- uniRapR
- Zdk
- Z-lock
Methods articles
Software
Dyes, site-specific labeling, small molecules
- dyes
- dyes in biosensors
- labeling, in cells and out
- small molecules other than dyes
- physical chemistry of dyes