hahn lab



For a complete list of publications click here.

Cell biology and physiology
Engineering allosteric networks in proteins
Image analysis, quantitative imaging, and microscopy
Small molecules to visualize and control proteins

Some key papers

Dagliyan, O., Tarnawski, M., Chu, P-H., Shirvanyants, D., Schlichting, I., Dokholyan, N.V., and Hahn, K.M. Engineering extrinsic disorder to control protein activity in living cells.  Science, 354(6318): 1441-1444, 2016. View free in Science online: Full text | Abstract | Reprint.

Hodgson, L., Spiering, D., Sabouri-Ghomi, M., DerMardirossian, C., Danuser, G., and Hahn, K.M. FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nature Chem. Biol., 12(10): 802-9, 2016. PMC5030135. Online article.

Wang, W., Vilela, M., Winkler, A., Tarnawski, T.,  Schlichting, I., Yumerefendi, H., Kuhlman, B., Liu, R., Danuser, G., and Hahn, K.M. LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation. Nature Methods, 13(9): 755-8, 2016. Online article.

Hayashi-Takagi, A., Yagishita, S., Nakamura, M., Shirai, F., Wu, Y.I., Loshbaugh, A.L., Kuhlman, B., Hahn, K.M., and Kasai, H. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 525(7569): 333-8, 2015.  PMC4634641. Online article.

Yi, J.J., Berrios, J., Newbern, J.M., Snider, W.D., Philpot, B.D., Hahn, K.M., and Zylka, M.J. An Autism-Linked Mutation Disables Phosphorylation Control of UBE3A. Cell, 162(4):795-807, 2015. PMC4537845. Online article.

Chu, P-H., Tsygankov, D., Berginski, M.E., Dagliyan, O., Gomez, S.M., Elston, T.C., Karginov, A.V., and Hahn, K.M. Engineered kinase activation reveals unique morphodynamic phenotypes and associated trafficking for Src family isoforms. Proc. Natl. Acad. Sci. U.S.A. 111(34):12420–12425, 2014. PMC4151743. Online article.

Yi, J.J., Wang, H., Vilela, M., Danuser, G., and Hahn, K.M. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth. Biol., 3(11):788-95, 2014. PMC4277778. Online article.

Karginov, A., Tsygankov, D., Berginski, M., Chu, P-H., Trudeau, E., Yi, J.J., Gomez, Shawn, Elston, T.C. and Hahn, K.M. Dissecting motility signaling through activation of specific Src-effector complexes. Nature Chem. Bio., 10(4):286-90, 2014.  PMC4064790. Online article.

Dagliyan, O., Shirvanyants, D., Karginov, A.V., Ding, F., Fee, L., Chandrasekaran, S.N., Freisinger, C.M., Smolen, G.A., Huttenlocher, A., Hahn, K.M., Dokholyan, N.V. Rational design of a ligand-controlled protein conformational switch. Proc. Natl. Acad. Sci. U.S.A., 110(17):6800-4, 2013. PMC3637791. Online article.

Lungu, O.I., Hallett R.A., Choi E.J., Aiken M.J., Hahn K.M.*, Kuhlman B.* Designing Photoswitchable Peptides Using the AsLOV2 Domain. Chemistry and Biology, 19(4):507-17, 2012. PMC334866. Online article.

Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston T., and Hahn, K.M.  A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem. Biol., 7: 437-444, 2011.  PMC3135387. Online article.

Wang, X., He L, Wu, Y.I., Hahn, K.M., and Montell, D.J. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nature Cell Biol., 12(6):591-7, 2011. PMC2929827. Online article.

Yoo, S.K., Deng, Q.,  Cavnar, P. J.,  Wu,Y.I.,  Hahn, K.M., Huttenlocher, A. Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish.  Developmental Cell, 18(2):226-236, 2010. PMC2824622. Online article.

Karginov, A.V., Ding, F., Kota, P., Dokholyan, N.V., and Hahn, K.M. Engineered allosteric activation of kinases in living cells. Nature Biotech, July; 28(7):743-7, 2010. PMC2902629. Online article.

Wu, Y, Frey, D., Lungu, O. I., Jaehrig, A., Schlichting, I., Kuhlman, B. and Hahn, K.M. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 461:104-110, 2009. PMC2766670. Online article.

Machacek, M., Hodson, L., Welch, C., Elliot, H., Pertz, O., Nalbant, P., Abell, A., Johnson, G., Hahn, K.M. and Danuser, G. Coordination of Rho GTPase activities during cell protrusion. Nature, 461:99-103, 2009. PMC2885353. Online article.

Toutchkine, A., Han, W.G., Ullmann, M., Liu, T., Bashford, D., Noodleman, L., and Hahn, K.M. Experimental and DFT studies: novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes. J. Phys. Chem. A., 111:10849-60, 2007. PMC3742023. Online article.

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Merocyanine dyes with improved photostability. Org. Lett., 9: 2775-2777, 2007. Online article.

Birkenfeld, J., Nalbant, P., Bohl, B.P., Pertz, O.P., Hahn, K.M., and Bokoch, G.M. GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases. Developmental Cell. 12:699-712, 2007. PMC1965589. Online article.

Pertz, O., L. Hodgson, R. R. Klemke, and K.M. Hahn. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature,  440(7087):1069-1072, 2006. Online article.

Subauste, M., O. Pertz, E. Adamson, C. E. Turner, S. Junger, K. M. Hahn. Vinculin modulation of Paxillin-FAK interactions regulates ERK to control survival and motility. J. Cell Biol., 165:171-181, 2004. PMC2172187. Online article.

Nalbant, P., L. Hodgson, V. Kraynov, A. Toutchkine, K.M. Hahn. Activation of Endogenous Cdc42 Visualized in Living Cells. Science, 305:1615-1619, 2004. Online article.

Toutchkine, A., V. Kraynov, and K.M. Hahn. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living Cells. J. Amer. Chem. Soc., 125:4132-4145, 2003. Online article.

Del Pozo, M.A., W.B. Kiosses, N.B. Alderson, N. Meller, K.M. Hahn, and A. Schwartz. Integrin-regulated membrane translocation of GTP-Rac induces localized interaction with effectors due to dissociation of RhoGDI. Nature Cell Biol., 4:232-239, 2002. Online article.

Kraynov, V. S., Chamberlain, C. E., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., and K. M. Hahn. Localized Rac activation dynamics visualized in living cells. Science, 290:333-337, 2000. Online article.

Subauste, C. M., M. Von Herrath, V. Benard, C. E. Chamberlain, T. H. Chuang, K. Chu, G. M. Bokoch, and K.M. Hahn. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and fas. J. Biol. Chem., 275(3):9725-9733, 2000. Online article.

Hahn, K.M., R. DeBiasio and D.L. Taylor.  Patterns of elevated free calcium and calmodulin activation in living cells. Nature, 359:736-738, 1992.

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