hahn lab

cell cinema

Movies chosen simply because they are fun to watch.

Optogenetics

moving laser causes cell protrusions

Moving laser spot induces Rac1 activation and consequent cell protrusion

by Yi Wu
Nature, 46:104-110, 2009

Pulses of light on one spot along the cell edge (red circle) activate Rac1 locally, producing first ruffles and then a protrusion

by Yi Wu
Nature, 461:104-110, 2009

HEK293 cell follows a light spot that activates Rac1

by Yi Wu
Nature, 461:104-110, 2009

Zebrafish spelling Rac - wiggly shaped letters R, A and C.

Fluorescent neutrophils in living zebrafish spelling "Rac"

by our collaborators, Sa Kan Yoo and Qing Deng, in the Huttenlocher Lab
Dev. Cell, 18:226-23, 2010

blue cell with with green a nucleus surrounded by red theny yellow then green then blue bands of color against a black background.

NIGMS Biomedical Beats: "Cool video: Leading Cells with light"

with captions and narration by NIH, images by Yi Wu
NIGMS Biomedical Beats, Sept, 2009

LOVETRAP reversible sequestration of GFP at mitochondria

by Hui Wang
Nature Methods, 13:755-758, 2016

LOVTRAP activation of VaV2 produces cell oscillations

by Hui Wang
Nature Methods, 13:755-758, 2016

3 images of a cell showing Rac1 photo inhibition

Rac1 photo-inhibition

by Onur Dagliyan
Science, 13:755-758, 2016

Vav2 photo-inhibition

by Onur Dagliyan
Science, 13:755-758, 2016

Optogenetic control of nuclear import

Developed by our collaborators in the Kuhlman Lab
Nature Chem. Biol., 12(6): 399-401, 2016.

Biosensors (Genetically encoded, HALO, SNAP, Binder/Tag…)

RhoA activation versus localization in a moving fibroblast

by Olivier Pertz and Louis Hodgson
Nature, 440:1069-1072, 2006

Rac1 activation in a motile fibroblast

by Christopher Welch
Nature, 461:104-110, 2009

Activation of Rap1 in dorsal ruffles. 3D analysis using ImageTank

by Ellen O'Shaughnessy
J. Cell Biol., 218(9):3153-3160, 2019

blue cell with a yellow and green nucelus with many purple.

GEF-H1 Microtubules (L) control GEF-H1 activity (R)

by Mihai Azoitei.
J. Cell Biol., 218 (9):3077–3097, 2019

Images of individual molecues colored red, green and yellow in a living cell. The yellow is the Src molecule which shows a yellow biosensor when in its open conformation.

Conformational changes of individual molecules within living cells. Using binder/tag, Src molecules turn yellow when in open conformation.

by Bei Liu
Cell, 2021:Oct11; doi.org/10.1016/j.cell.2021.09.026

A blue cell with dots of green, dark blue and yellow, mostly along the cell edge, which are proteins with biosensors attached,  that change color as the proteins within the cell, do different activities which cause its conformation to change.

Simple engineering of FRET biosensor using binder/tag. Src activation during cell motility.

by Bei Liu
Cell, 2021:Oct11; doi.org/10.1016/j.cell.2021.09.026

Biosensors based on environment-sensing dyes

Cdc42 activation in RhoA-inhibited cell; meroCBD biosensor

by Perihan Nalbant and Louis Hodgson
Science, 305:1615-1619, 2004

protein structure of phospho-ERK2 with merocyanine dye attached

Merocyanine dye on phospho-ERK2

by Alexei Toutchkine and Brenda Temple

blue cell with many colored biosensors around the edge of the cell indicating Src activation in macropinocytic vesicles.

Src activation in macropinocytic vesicles.

by Akash Gulyani
Nature Chem Biol, 7(7):437-44, 2011

Image analysis and modeling

Sampling windows track the cell edge for localized assessment of biosensor activity

Developed by our collaborators in the Danuser lab.
Nature, 461:99-103, 2009

orange cell with automated line tracking graph next to it

Automated tracking of cell morphology over time. Simple 2D trace captures morphodynamics over time

Developed by our collaborators in the Elston and Tsygankov labs.
PNAS, 111(34):12420–12425, 2014

CellGeo package accurately measures dimensions and morphodynamics of complex cell boundaries

Developed by our collaborators in the Elston and Tsygankov labs.
J. Cell Biol., 204(3):443-460, 2014

Analysis of coordinate GEF-H1 and cell protrusive activity. Analysis by Danuser lab

by Mihai Azoitei.
J. Cell Biol., 218(9):3077–3097, 2019

Rap1 activity bridges ventral ECM interactions and dorsel ruffles. 3D visualization using ImageTank software

by Ellen O'Shaughnessy
J. Cell Biol., 218(9):3153-3160, 2019

3D view of Rap1 activation in moving fibroblast using ImageTank

by Ellen O'Shaughnessy
J. Cell Biol., 218(9):3153-3160, 2019

Engineered allosteric control of protein activity

3 cells showing activation by Rapr analogs of the Src family: Fyn, Src and Lyn.

RapR analogs of Src family kinases. Specific activation of the closely related family members Fyn, Src, and Lyn produce different cell phenotypes.

These 3 videos show: top - Fyn (spreading); middle - Src (spreading followed by movement); bottom - Lyn (suprising kinked extensions).

by Pei Hsuan Chu
PNAS, 111(34):12420-25, 2014

cell showing activation of RapR-Src generates cells preading and adheasion dynamics

Activation of RapR-Src generates cell spreading and adhesion dynamics

by Andrei Karginov
Nature Chem Biol, 10(4):286-90, 2014

a cell

Photo-inhibition of Src kinase

by Onur Dagliyan
Science, 354(6318):1441-1444, 2016

2 cells

Inhibition of GEFs with light

by Onur Dagliyan
Science, 354(6318):1441-1444, 2016

cell

Multiplexed optogenetic control, using photo-activatable Vav2 (PA-Vav2) and photo-inhibitable Rac1 (PI-Rac1) in the same cell

by Onur Dagliyan
Science, 354(6318):1441-1444, 2016

Miscellaneous

Cytotoxic lymphocyte killing a virally infected fibroblast (oldie but goodie)

by Klaus Hahn, Robin DeBiasio, Greg LaRocca, D. Lansing Taylor, and Michael Oldstone
Virology, 201(2):330-340, 1994

2 green colored cells have bright round areas moving around inside them

GFP Vinculin reveals adhesion dynamics and dorsal ruffles

by Louis Hodgson

Retrograde Transport

by Noah Sciaky

Tiled DIC movies of moving fibroblast

by Femo Shen


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~ Updated 02/02/2022

© UNC Department of Pharmacology