Macrophage forms a ring of podosomes (purple dots) when contacting a micro patterned circle of substrate antibodies (green).

By Shiqiong Hu, Takashi Watanbe and Bei Liu.
PLoS Comput. Biol., 18(10):e1010092, 2022.
Copyright Hahn Lab UNC Chapel Hill

Primary mouse macrophage expressing FcγRIIA-EGFP (green) and LifeactmCherry (magenta) migrates over a 15 μm IgG disk, forming podosomes around the edge of the disk. The images were acquired by confocal microscopy at 5 seconds intervals.