Applications from curious and creative scientists with diverse backgrounds are welcome. Please send a CV and cover letter outlining your research interests and goals to khahn[at]med.unc.edu. We are specifically seeking:
- Postdoctoral fellows with experience in single molecule imaging, to explore new methods for visualizing conformational changes of individual molecules within living cells. We will study the nanometer scale structural dynamics of integrin adhesion complexes, podosomes, and potentially other dynamic organelles. The role of these structures in phagocytosis, mechanotransduction, and metastasis controlled by collagen alignment will be explored. This project is a close collaboration with the Elston lab at UNC, who will work with you for quantitative image analysis and to model oscillating signaling networks. You will work together with chemists and other scientists in our lab to generate new biosensors and optogenetic proteins. Development of microscopes for single particle tracking or lattice light sheet imaging in conjunction with atomic force microscopy, together with the Superfine lab at UNC, could be an important aspect of this work.
- We were recently funded to develop novel biosensor and optogenetic approaches that can be combined to manipulate and visualize protein activities in the same cell. We seek protein engineers/biochemists/molecular biologists to develop broadly applicable methods and apply them to specific proteins in GTPase pathways. Morphodynamics will be a model system explored to elucidate cytoskeletal and adhesion control of GTPase signaling. This project is a partnership with our long-standing collaborator Gaudenz Danuser of UTSW. Using biosensor data, Dr. Danuser’s analytical approaches can extract causal connections between protein activation events.
- We seek organic and physical organic chemists, to study and develop novel environment-sensing dyes and protein-dye conjugates, including structures for single molecule imaging in vivo, and for multiplexed biosensor/optogenetic imaging. Projects here will focus on the GTPase cycle and on exocysts. We offer an opportunity for chemists to learn protein engineering and microscopy, and to apply their own new probes to answer cell biology questions. This project involves novel approaches for specific derivatization of proteins within live cells.
We also offer permanent appointments to develop new microscopes that complement the molecules produced in the above projects, and to code for microscope automation and image analysis (guided in part by Drs. Elston, Danuser or Superfine). Experience with 3D SIM, light sheet microscopes, single particle imaging, or microscope automation would be valuable. We are seeking technicians well versed in cloning, protein purification, imaging and/or biochemical assays.
~ Updated 10/13/2021