Microtubule trapped within a ring of podosomes (at approximately 5 o’clock)

By Shiqiong Hu, Takashi Watanbe and Bei Liu.
PLoS Comput. Biol., 18(10):e1010092, 2022.
Copyright Hahn Lab UNC Chapel Hill

RAW 264.7 macrophages expressing a GFP fusion of the ensconsin MT-binding domain (EMTBEGFP) to mark microtubules (cyan) and Lifeact-Halo-549 to mark F-actin (magenta). The duration of this TIRFSIM movie is 1 min and 18 seconds with 2 second intervals between frames. Note the MT restricted within a circle of actin (approx. 5 o’clock).