Multiplexed imaging of GTPase activation dynamics

by Daniel J. Marston
Nature Chem Biol. 16(8): 826–833, 2020.
Copyright Hahn Lab UNC Chapel Hill

Simultaneously imaged RhoGEF and Rho GTPase biosensors in MDA-MB-231 cells undergoing constitutive edge motion. Upper images show activation of Asef (left) and Cdc42 (right), lower images show Asef (left) and Rac1 (right).