LOV is photoactivated very rapidly (< 0.5 milliseconds). The half time for return to the dark state can be adjusted using point mutations (in our hands from 1.7 to 496 seconds, Curr. Protoc. Cell Biol., 73: 21.10.1-14, 2016). Rapid return times are useful for precise kinetic control. Slower return times enable continuous activation with only a brief pulse of light every few minutes.

References

LOVTRAP

Wang, W., Vilela, M., Winkler, A., Tarnawski, T.,  Schlichting, I., Yumerefendi, H., Kuhlman, B., Liu, R., Danuser, G., and Hahn, K.M. LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation. Nature Methods, 13(9): 755-8, 2016. PMC5137947. Online article | Free PMC article

Takano, T., Wu, M., Nakamuta, S., Naoki, H., Ishizawa, N., Namba, T., Watanabe, T., Xu, C., Hamaguchi, T., Yura, Y., Amano, M., Hahn, K.M. and Kaibuchi, K. Discovery of long-range inhibitory signaling to ensure 0.3 single axon formation. Nature Communications, 8(1): 33, 2017. PMC5484694. Online article | Free PMC article

Z-lock

Stone, O.J., Pankow, N., Liu, B., Sharma, V.P., Eddy, R.J., Wang, H., Putz, A.T., Teets, F.D.,  Kuhlman, B., *Condeelis, J.S., and *Hahn, K.M. Optogenetic control of Cofilin and aTAT in living cells using Z-lock. Nature Chemical Biology, 15: 1183-1190, 2019. PMC6873228. Online article | Free PMC article

Zdk

Van Haren, J., Charafeddine, R.A., Ettinger, A., Wang, H., Hahn, K.M., and Wittman, T. Local Control of Intracellular Microtubule Dynamics by EB1 Photo-Dissociation. Nature Cell Biol., 20(3):252-261, 2018.  
PMC5826794. Online article | Free PMC article

Methods articles

Wang, H. and Hahn, K.M. LOVTRAP: a versatile method to control protein function with light. Curr. Protoc. Cell Biol., 73: 21.10.1 - 21.10.14, 2016. PMC5137945. Online article | Free PMC article

 

 

~ Updated 04/09/2021