Zawistowski, J.S., Sabouri-Ghomi, M., *Danuser, G., *Hahn, K.M. and *Hodgson, L. A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. PLoS One, 8(11): e79877, 2013. PMC3818223. Online article | Free PMC article
Pertz, O., Hodgson, L., Klemke, R.R., and Hahn, K.M. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature, 440(7087):1069-1072, 2006. Online article | PDF
Hodgson, L., Pertz, O., and Hahn, K.M. Design and Optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol, 85:63-81, 2008. [Link downloads PDF] Online article
*Note: this older methods article contains a useful description of biosensor image analysis.
Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF
*Note: this article is focused on dye-based biosensors, but includes a method to correct for bleaching of any fluorophore.
~ Updated 04/12/2021