hahn lab

GTPase biology

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Herron, J.C., Hu, S., Liu, B., Watanabe, T., Hahn, K.M., and Elston, T.C. Spatial models of pattern formation during phagocytosis. PLoS Comput Biol. 18(10): e1010092, 2022. PMC9560619. Open Access online article | Free PMC article

Chadelle, L., Liu, J., Choesmel-Cadamuro, V., Karginov, A.V., Froment, C., Burlet-Schiltz, O., Gandarillas, S., Barreira, Y., Segura, C., Van Den Berghe, L., Czaplicki, G., Van Acker, N., Dalenc, F., Franchet, C., Hahn, K.M., Wang, X., and Belguise, K. PKCθ-mediated serine/threonine phosphorylations of FAK govern adhesion and protrusion dynamics within the lamellipodia of migrating breast cancer cells. Cancer Letters, 526:112-130, 2021. PMC9019305. Onine article | Free PMC article available 2/1/2023

Bianchi-Smiraglia, A., Wolff, D.W., Marston, D.J., Deng, Z.,  Moparthy, S., Wombacher, R.M., Mussel, AL.,  Shen, S., Yun, D-H., Cox, A.O., Furdue, C., Hurley, E., Feltri, M.L., Qu, J., Hollis, T., Kengne, J.B.N., Fongang, B., Sousa, R.J., Kandel, M.E., Kandel, E.S., Hahn, K.M., and Nikiforov, M.A. Regulation of local GTP availability controls RAC1 activity and cell invasion. Nature Communications, 12: 6091, 2021. PMC8526568. Online article |Free PMC article

Howell, M., Delorme-Walker, V., Welch, C., Pathak, R., Hahn, K.M., and DerMardirossian, C. Mechanistic of Rac and Cdc42 synchronization at the cell edge by ARHGAP39-dependent signaling nodules and the impact on protrusion dynamics. bioRxiv, May 13, 2021. doi: 10.1101/2021.05.13.443687 Online article

Popkova, A., Stone, O.J., Chen, L., Qin, X., Liu, C., Liu, J., Belguise, K., Montell, D.J., Hahn, K.M., Rauzi, M., and Wang, X. A Cdc42-mediated supracellular network drives polarized forces and Drosophila egg chamber extension. Nature Communications, 11(1): 1921, 2020. PMC7174421. Online article |Free PMC article

Marston, D.J., Vilele, M., Huh, J., Ren, J., Azoitei, M., Glekas, G., *Danuser, G., *Sondek, J., and *Hahn, K.M. Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis.. Nature Chem. Biol., 16(8): 826-833, 2020. PMC7388658. Online article | Free PMC article

O'Shaugnessy, E.C., Stone, O.J., LaFosse, P.K., Azoitei, M.L., Tsygankov, D., Heddleston, J.M., Legant, W.R., Wittchen, E.S., Burridge, K., Elston, T.C., Betzig, E., Chew, T.L., Adalsteinsson, D., and Hahn, K.M. Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor. J. Cell Biol., 218(9): 3153-3160, 2019. PMC6719445. Online article | Free PMC article

Azoitei, M.L., Noh, J., Marston, D.J., Roudot, P., Marshall, C.B., Daugird, T.A., Lisanza, S.L., Sandí, M.J., Ikura, M., Sondek, J., Rottapel, R., *Hahn, K.M., and *Danuser, G. Spatiotemporal dynamics of GEF-H1 activation controlled by microtubule- and Src-mediated pathways. J. Cell Biol., 218(9): 3077-3097, 2019. PMC6719461. Online article | Free PMC article

Marston, D.J., Anderson, K.L., Swift, M.F., Rougie, M., Page, C., Hahn, K.M., Volkmann, N., and Hanein, D. High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture. Proc. Natl. Acad. Sci. U.S.A., 116(4):1267-1272, 2019. PMC6347697. online article | Free PMC article

Stefanini, L., Lee, R.H., Paul, D.S., O'Shaughnessy, E.C., Ghalloussi, D., Jones, C.I., Boulaftali, Y., Poe, K.O., Piatt, R., Kechele, D.O., Caron, K.M., Hahn, K.M., Gibbins, J.M., and Bergmeier, W. Functional redundancy between RAP1 isoforms in murine platelet production and function. Blood, 132(18):1951-1962m 2018. PMC6213319. Online article | Free PMC article

Woroniuk, A., Porter, A., White, G., Newman, D.T., Diamantopoulou, Z., Waring, T., Rooney, C., Strathdee, D., Marston, D.J., Hahn, K.M., Sansom, O.J., Zech, T., and Malliri, A. STEF/TIAM2-mediated Rac1 activity at the nuclear envelope regulates the perinuclear actin cap. Nature Commun., 9(1):2124, 2018. PMC5974301. Online article | Free PMC Article

Tajadura-Ortega, V., Garg, R., Allen, R., Owczarek, C., Bright, M.D., Kean, S., Mohd-Noor, A., Grigoriadis, A., Elston, T.C., Hahn, K.M., Ridley, A.J. An RNAi screen of Rho signalling networks identifies RhoH as a regulator of Rac1 in prostate cancer cell migration. BMC Biol., 16(1):29, 2018. PMC5840776. Online article | Free PMC article

Takano, T., Wu, M., Nakamuta, S., Naoki, H., Ishizawa, N., Namba, T., Watanabe, T., Xu, C., Hamaguch i, T., Yura, Y., Amano, M., Hahn, K.M. and Kaibuchi, K. Discovery of long-range inhibitory signaling to ensure 0.3 single axon formation. Nature Communications, 8(1):33, 2017. PMC5484694. Online article | Free PMC article

Herrington, K.A., Trinh, A.L., Dang, C., O'Shaughnessy, E., Hahn, K.M., Gratton, E., Digman, M.A. and Sütterlin, C. Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol. Biol. Cell, 28(15): 2135-2145, 2017. PMC5509425. Online article | Free PMC article

Woodham, E.F., Paul, N.R., Tyrrell, B., Spence, H.J., Swaminathan, K., Scribner, M.R., Giampazolias, E., Hedley, A., Clark, W., Kage, F., Marston, D.J., Hahn, K.M., Tait, S.W., Larue, L., Brakebusch, C.H., Insall, R.H. and Machesky, L.M. Coordination by Cdc42 of actin, contractility, and adhesion for melanoblast movement in mouse skin. Curr. Biol., 27(5): 624-637, 2017. PMC5344686. Online article | Free PMC article

*Hodgson, L., *Spiering, D., Sabouri-Ghomi, M., Dagliyan, O., DerMardirossian, C., *Danuser, G. and *Hahn, K.M. FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nature Chem. Biol., 12(10): 802-9, 2016. PMC5030135. Online article | Free PMC article

Nguyen, T.T., Park, W.S., Park, B.O., Kim, C.Y., Oh, Y., Kim, J.M., Choi, H., Kyung, T., Kim, C.H., Lee, G., Hahn, K.M., Meyer, T. and Heo, W.D. PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front. Proc. Natl. Acad. Sci. U.S.A., 113(36): 10091-6, 2016. PMC5018766. Online article | Free PMC article

Hayashi-Takagi, A., Yagishita, S., Nakamura, M., Shirai, F., Wu, Y.I., Loshbaugh, A.L., Kuhlman, B., Hahn, K.M., and Kasai, H. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 525(7569): 333-8, 2015.  PMC4634641. Online article | Free PMC article

Khalil, B.D., Hanna, S., Saykali, B.A., El-Sitt, S., Nasrallah, A., Marston, D., El-Sabban, M., Hahn, K.M., Symons, M. and El-Sibai, M. The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility. Exp. Cell Res., 321(2): 109-122, 2014. PMC4297755. Online article | Free PMC article

Zawistowski, J.S., Sabouri-Ghomi, M., Danuser, G., *Hahn, K.M., and *Hodgson, L. A RhoC Biosensor Reveals Differences in the Activation Kinetics of RhoA and RhoC in Migrating Cells. PLoS One 8(11): e79877, 2013. PMC3818223. Online article | Free PMC article

Samson, T., van Buul, J.D., Kroon, J., Welch, C., Bakker, E.N., Matlung, H.L., van den Berg, T.K., Sharek, L., Doerschuk, C., Hahn, K. and Burridge, K. The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis. PLoS One, 8(1): e55202, 2013. PMC3555862. Online article | Free PMC article

Collins, C., Guilluy, C., Welch, C., O’Brien, E.T., Hahn, K.M., Superfine, R., Burridge, K. and Tzima, E.  Localized tensional forces on PECAM-1 elicit a global mechanotransduction response via the integrin-RhoA pathway. Curr. Biol., 22(22): 2087-2094, 2012. PMC3681294. Online article | Free PMC article

Dietz, D.M., Sun, H., Lobo, M.K., Cahill, M.E., Chadwick, B., Gao, V., Koo, J.W., Mazei-Robison, M.S., Dias, C., Maze, I., Damez-Werno, D., Dietz, K.C., Scobie, K.N., Ferguson, D., Christoffel, D., Ohnishi, Y., Hodes, G.E., Zheng, Y., Neve, R.L., Hahn, K.M., Russo, S.J. and Nestler, E.J. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nature Neurosci., 15(6): 891-896, 2012.  PMC3565539. Online article | Free PMC article

Wu, Y.I., Wang, X., He, L., Montell, D. and Hahn, K.M. Spatiotemporal control of small GTPases with light using the LOV domain. Methods Enzymol., 497: 393-407, 2011.  PMC3407667. Online article | Free PMC article

Helfand, B.T., Mendez, M.G., Murthy, S.N.P., Shumaker, D.K., Grin, B., Mahammad, S., Ueli Aebi, U., Wedig, T., Wu, Y.I., Hahn, K.M., Inagaki, M., Herrmann, H. and Goldman, R.D.  Vimentin organization modulates the formation of lamellipodia. Mol. Biol. Cell, 22: 1274-1289, 2011. PMC3078081. Online article | Free PMC article

Samson, T., Welch, C.M., Monaghan-Benson, E., Hahn, K.M. and Burridge, K. Endogenous RhoG is rapidly activated after EGF stimulation through multiple GEFs. Mol. Biol. Cell, 21(9): 1629-1642, 2010. PMC2861620. Online article | Free PMC article

Wang, X., He L, Wu, Y.I., Hahn, K.M., and Montell, D.J. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nature Cell Biol., 12(6):591-7, 2011. PMC2929827. Online article | Free PMC article

Koivusalo, M., Welch, C., Hayashi, H., Scott, C.C., Kim, M., Alexander, T., Touret, N., Hahn, K.M., and Grinstein, S. Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signalling. J. Cell Biol., 188: 547-563, 2010. PMC2828922. Online article | Free PMC article

Yoo, S.K., Deng, Q.,  Cavnar, P. J.,  Wu,Y.I.,  Hahn, K.M., Huttenlocher, A. Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish.  Developmental Cell, 18(2):226-236, 2010. PMC2824622. Online article | Free PMC article

Wu, Y, Frey, D., Lungu, O. I., Jaehrig, A., Schlichting, I., Kuhlman, B. and Hahn, K.M. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 461:104-108, 2009. PMC2766670. Online article | Free PMC article

Machacek, M., Hodson, L., Welch, C., Elliot, H., Pertz, O., Nalbant, P., Abell, A., Johnson, G., *Hahn, K.M. and *Danuser, G. Coordination of Rho GTPase activities during cell protrusion. Nature, 461: 99-103, 2009. PMC2885353. Online article | Free PMC article

Su, L., Pertz, O., Mikawa, M., Hahn, K., and Parsons, S.J. p190RhoGAP negatively regulates Rho activity at the cleavage furrow of mitotic cells. Exper.Cell Res., 315: 1347-59, 2009. PMC273142. Online article | Free PMC article

Manukyan, M., Nalbant, P., Hahn, K.M., and Knaus, U.G. RhoA GTPase activation by TLR ligands: connecting via Src to NF-kB. J. Immunol. 182(6):3522-3529, 2009. PMC2684960. Online article | Free PMC article

El-Sibai, M., Pertz, O., Pang, H., Yip, S.C., Lorenz, M., Symons, M., Condeelis, J.S., Hahn, K.M. and Backer, J.M. RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells. Exp. Cell Res. 314: 1540-1552, 2008. PMC2677995. Online article | Free PMC article

Healy, K.D., Hodgson, L., Kim, T.Y., Shutes, A., Maddileti, S., Juliano, R.L., Hahn, K.M., Harden, T.K., Bang, Y.J. and Der, C.J. DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms. Mol. Carcinog., 47(5): 326-337, 2008. PMC2679972. Online article | Free PMC article

Frantz, C., Karydis, A., Nalbant, P., Hahn, K.M. and Barber, D.L.  Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells.  J. Cell Biol., 179: 403-410, 2007. PMC2064788. Online article | Free PMC article

El-Sibai, M., Nalbant, P., Sarmiento, C., Macaluso, F., Cammer, M., Condeelis, J.S., Hahn, K.M. and Backer, J.M. Cdc42 is required for EGF-stimulated protrusion and motility in carcinoma cells. J. Cell Science, 120(Pt 19): 3465-3474, 2007. PMC4066376. Online article | Free PMC article

Hodgson, L., Chan, E.W.L., Hahn, K.M., and Yousaf, M.N. Combining Surface Chemistry with a FRET-Based Biosensor to Study the Dynamics of RhoA GTPase Activation in Cells on Patterned Substrates. J. Am. Chem. Soc. 129:9264 - 9265, 2007. Online article | PDF

Birkenfeld, J., Nalbant, P., Bohl, B.P., Pertz, O.P., Hahn, K.M. and Bokoch, G.M. GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases. Dev. Cell, 12: 699-712, 2007. PMC1965589. Online article | Free PMC article

Van Keymeulen, A., Wong, K., Knight, Z.A., Govaerts, C., Hahn, K.M., Shokat, K.M. and Bourne, H.R. To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front. J. Cell Biol., 174: 437-445, 2006. PMC2064239. Online article | Free PMC article

Wong, K., Pertz, O., Hahn, K. and Bourne, H. Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism. Proc. Natl. Acad. Sci. U.S.A., 103: 3639-3644, 2006. PMC1450135. Online article | Free PMC article

Pertz, O., Hodgson, L., Klemke, R.R., and Hahn, K.M. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature,  440(7087):1069-1072, 2006. Online article | PDF

Shen, F., Hodgson, L., Rabinovich, A., Pertz, O., Hahn, K.M. and Price, J. H. Functional proteomics for cell migration. Cytometry, 69: 563-572, 2006. Online article

Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A. and Hahn, K.M. Activation of endogenous Cdc42 visualized in living cells. Science, 305: 1615-1619, 2004. Onilne article | PDF

Tu, S.S., Wen, J.W., Yang, W., Nalbant, P., Hahn, K.M. and Cerione, R.A. Anti-apoptotic Cdc42 mutants are potent activators of cellular transformation. Biochemistry, 41: 12350-12358, 2002. Online article | PDF

Gardiner, E.M., Pestonjamasp, N., Bohl, B.P., Chamberlain, C., Hahn, K.M. and Bokoch, G.M. Spatial and temporal analysis of Rac activation during live neutrophil chemotaxis:  Roles in leading edge extension and tail retraction. Curr. Biol., 12: 2029-2034, 2002. Online article | PDF

Katsumi, A., Milanini, J., Kiosses, W.B., del Pozo, M.A., Kaunas, R., Chien, S., Hahn, K.M. and Schwartz, M.A. Effects of cell tension on membrane protrusion through regulation of the GTPase Rac. J. Cell Biol., 158: 1-13, 2002. PMC2173027. Online article | Free PMC article

Del Pozo, M.A., Kiosses, W.B., Alderson, N.D., Meller, N., Hahn, K.M. and Schwartz, M.A.  Integrin regulated membrane translocation of GTP-Rac induces localized interaction with effectors due to dissociation of RhoGDI. Nature Cell Biol., 4: 232-239, 2002. Online article | PDF

Kraynov, V.S., Chamberlain, C.E., Bokoch, G.M., Schwartz, M. A., Slabaugh, S. and Hahn, K.M. Localized Rac activation dynamics visualized in living cells.  Science, 290: 333-337, 2000. Online article | PDF

Subauste, C.M., Von Herrath, M., Benard, V., Chamberlain, C.E., Chuang, T.H., Chu, K., Bokoch, G.M. and Hahn, K.M. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and fas. J. Biol. Chem., 275 (3): 9725-9733, 2000. Online article | PDF

Ben-Ami, G., Ozeri, V., Hanski, E., Hofmann, F., Aktories, K., Hahn, K.M., Bokoch, G.M. and Rosenshine, I. Agents that inhibit Rho, Rac and Cdc42 do not block formation of actin pedestals in HeLa cells infected with enteropathogenic Escherichia coli. Infect. Immun., 66: 1755-1758, 1998. PMC108116. Online article | Free PMC article

Chuang, T.H., Hahn, K.M., Lee, J.D., Danley, D.E. and Bokoch, G.M. The small GTPase Cdc42 initiates an apoptotic signaling pathway in Jurkat T lymphocytes. Mol. Biol. Cell, 8: 1687-1698, 1997. PMC305729. Online article | Free PMC article

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