Mehl, B.P., Vairaprakash, P., Li, L., Hinde, E., MacNevin, C.J., Hsu, C.W., Gratton, E., Liu, B., and Hahn, K.M. Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes. Cell Rep Methods. 4(3): 100734, 2024. PMC10985238. Online article | Free PMC article
Pinkin, N.K., Liu, B., Pimenta, F.M., and Hahn, K.M. Using SNAP-tag for facile construction of dye-based biosensors in living cells. bioRxiv, July 16, 2020. doi: 10.1101/2020.07.16.206748. Online article
MacNevin, C.J., Watanabe, T., Weitzman, M., Gulyani, A., Fuehrer, S., Pinkin, N.K., Tian, X., Liu, F., Jin, J., and Hahn, K.M. Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells. J. Am. Chem. Soc., 141(18): 7275-7282., 2019. PMC6572722. Online article | Free PMC article
Ye, F., Yang, C., Kim, J., MacNevin, C.J., Hahn, K.M., Park, D., Ginsberg, M.H., and Kim, C. Epigallocatechin Gallate Has Pleiotropic Effects on Transmembrane Signaling by Altering the Embedding of Transmembrane Domains. J. Biol. Chem., 292(24): 2858-2864, 2017. PMC5473239 Online article | Free PMC article
MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V., and Hahn, K.M. Ratiometric imaging using a single dye enables simultaneous visualization of Rac1 and Cdc42 activation. J. Am. Chem. Soc., 138(8): 2571-2575, 2016. PMC4825053. Online article | Free PMC article
Helassa, N., Garnett, J.P., Farrant, M., Khan, F., Pickup, J.C., Hahn, K.M., MacNevin, C.J., Tarran, R., and Baines, D.L. A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochem. J., 464(2):213-20, 2014. PMC4357280. Online article | Free PMC article
Konze, K.D., Ma, A., Li, F., Barsyte-Lovejoy, D., Parton, T., MacNevin, C.J., Liu, F., Gao, C., Huang, X.P., Kuznetsova, E., Rougie, M., Jiang, A., Pattenden, S.G., Norris, J.L., James, L.I., Roth, B.L., Brown, P.J., Frye, S.V., Arrowsmith, C.H., Hahn, K.M., Wang, G.G., Vedadi, M. and Jin, J. An orally bioavailable chemical probe of the lysine methyltransferases EZH2 and EZH1. ACS Chem. Biol., 8(6): 1324–1334, 2013. PMC3773059. Online article | Free PMC article
Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., and Plückthun, A. Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells. Chem. Biol., 20(6): 847-56, 2013. PMC4154710. Online article | Free PMC article
MacNevin, C.J., Gremyachinskiy, D., Hsu, C.W., Li L., Rougie, M., Davis, T.T., Hahn, K.M. Environment-sensing merocyanine dyes for live cell imaging applications. Bioconjug. Chem., 24(2):215-23, 2013. PMC3667669. Online article | Free PMC article
Jha, R.K., Wu, Y.I., Zawistowski, J., MacNevin, C., *Hahn, K.M. and *Kuhlman B. Redesign of the PAK1 auto-inhibitory domain for enhanced stability and affinity in biosensor applications. J. Mol. Biol., 413(2): 513-522, 2011. PMC3202338. Online article | Free PMC article
Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston, T. and Hahn, K.M. A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem. Biol., 7: 437-444, 2011. PMC3135387. Online article | Free PMC article
Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476. Online article | Free PMC article
Toutchkine, A., Han, W.G., Ullmann, M., Liu, T., Bashford, D., Noodleman, L., and Hahn, K.M. Experimental and DFT studies: novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes. J. Phys. Chem. A, 111:10849-60, 2007. PMC3742023. Online article | Free PMC article
Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Merocyanine dyes with improved photostability. Org. Lett., 9: 2775-2777, 2007. Online article | PDF
Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Simple one-pot preparation of water-soluble, cysteine-reactive cyanine and merocyanine dyes for biological imaging. Bioconjugate Chem., 18:1344-1348, 2007. PMC3694264. Online article | Free PMC article
Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF
Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A., and Hahn, K.M. Activation of endogenous Cdc42 visualized in living cells. Science, 305: 1615-1619, 2004. Onilne article | PDF
Liu, T., Han, W., Himo, F., Ullmann, G.M., Bashford, D., Toutchkine,A., Hahn, K.M., and Noodleman, L. Density Functional Vertical Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A, 108: 3545-3555, 2004. online article | PDF
Tan, X., Nalbant, P., Toutchkine, A., Hu, D., Vorpagel, E.R., Hahn, K.M. and Lu, H.P. Single molecule study of protein-protein interaction dynamics in a cell signaling system. J. Phys. Chem. B, 108: 737-744, 2004. online article | PDF
Han, W. G., Liu, T., Himo, F., Toutchkine, A., Bashford, D., Hahn, K.M., and Noodleman, L. A Theoretical Study of the UV Absorption and Emission Solvatochromic Properties of Solvent Sensitive Dyes. ChemPhysChem, 4:1084-1094, 2003. Online article | PDF
Toutchkine, A., Kraynov, V., and Hahn, K.M. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living Cells. J. Amer. Chem. Soc., 125:4132-4145, 2003. Online article | PDF
Toutchkine, A., Nalbant, P., and Hahn, K.M. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility. Bioconjugate Chem., 3:386-389, 2002. Onine article | PDF
Bark, S. J., Schmid, S., and Hahn, K.M. A highly efficient method for site specific modification of peptides after chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000. Online article | PDF
Bark, S. J., and Hahn, K.M. Fluorescent indicators of peptide cleavage inside living cells. Methods, 20: 429-435, 2000. Online article | PDF
Cornish, V. W., Hahn, K.M., and Schultz. P.G. Site-specific protein modification using a ketone handle. J. Am. Chem. Soc., 118: 8150-8151, 1996. Online article | PDF
Knauf, P.A., Law, F.Y., and Hahn, K.M. An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange. Am. J. Physiol. (Cell Physiol.), C1073-C1077, 1995. Online article | PDF
Southwick, P., Hahn, K.M., Chao, J., Parry, P., Wagman, A.S., Wagner, M, and Waggoner, A.S. A long wavelength biolabeling reagent based on the oxonol flurophore. J. Fluorescence, 5(2): 231-235, 1995. Online article | PDF
Hahn, K.M., Conrad, P.A., Chao, J., Taylor, D.L., and Waggoner, A.S. A photocross-linking, fluorescent indicator of mitochondrial membrane potential. J. Histochem. Cytochem., 41: 631-634, 1993. Online article | PDF
Hahn, K.M., DeBiasio, R. and Taylor, D.L. Patterns of elevated free calcium and calmodulin activation in living cells. Nature, 359: 736-738, 1992. Online article | PDF
Hahn, K.M., Hastie, S.B. and Sundberg, R.J. Synthesis and evaluation of 2 diazo 3,3,3 trifluoropropanoyl derivatives of colchicine and podophyllotoxin as photoaffinity labels: reactivity, photochemistry, and tubulin binding. Photochem. Photobiol., 55: 17-27, 1992. Online article | PDF
Hahn, K.M. and Southwick, P.L. A stable diazo photoaffinity label with increased absorptivity and effective photoactivation beyond 300 nm. Anal. Biochem., 196: 271-278, 1991. Online article | PDF
Hahn, K.M., Humphries, W.G., Helms, A.M., Hastie, S.B. and Macdonald, T.L. Structural requirements for the binding of colchicine analogs to tubulin: the role of the C-10 substituent. Biomed. Chem. Lett., 1: 471-476, 1991. PDF
Hahn, K.M., Waggoner A.S., and Taylor, D.L. A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin- binding fluorophore. J. Biol. Chem., 265: 20335-20345, 1990. Online article | PDF
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