Mehl, B.P., Vairaprakash, P., Li, L., Hinde, E., MacNevin, C.J., Hsu, C.W., Gratton, E., Liu, B., and Hahn, K.M. Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes. Cell Rep Methods. 4(3): 100734, 2024. PMC10985238. Online article | Free PMC article
Pimenta, F.M., Huh, J., Guzman, B., Amanah, D., Marston, D.J., Pinkin, N.K., Danuser, G., and Hahn, K.M. Rho MultiBinder, a fluorescent biosensor that reports the activity of multiple GTPases. Biophys. J. 122(18): 3636-3655, 2023. PMC10541480. Online article | Free PMC article (available on 2024-09-19)
Bianchi-Smiraglia, A., Wolff, D.W., Marston, D.J., Deng, Z., Moparthy, S., Wombacher, R.M., Mussel, AL., Shen, S., Yun, D-H., Cox, A.O., Furdue, C., Hurley, E., Feltri, M.L., Qu, J., Hollis, T., Kengne, J.B.N., Fongang, B., Sousa, R.J., Kandel, M.E., Kandel, E.S., Hahn, K.M., and Nikiforov, M.A. Regulation of local GTP availability controls RAC1 activity and cell invasion. Nature Communications, 12: 6091, 2021. PMC8526568. Online article |Free PMC article
Liu, B., Stone, O.J., Pablo, M., Herron, C.J., Nogueira, A.T., Dagliyan, O., Grimm, J.B., Lavis, L.D., Elston, T.C., and Hahn, K.M. Biosensors based on peptide exposure show single molecule conformations in live cells. Cell, 184(22): 5670-5685, 2021. PMC8556369. Online article | Free PMC article
Pinkin, N.K., Liu, B., Pimenta, F.M., and Hahn, K.M. Using SNAP-tag for facile construction of dye-based biosensors in living cells. bioRxiv, July 16, 2020. doi: 10.1101/2020.07.16.206748. Online article
Howell, M., Delorme-Walker, V., Welch, C., Pathak, R., Hahn, K.M., and DerMardirossian, C. Mechanistic of Rac and Cdc42 synchronization at the cell edge by ARHGAP39-dependent signaling nodules and the impact on protrusion dynamics. bioRxiv, May 13, 2021. doi: 10.1101/2021.05.13.443687 Online article
Marston, D.J., Vilele, M., Huh, J., Ren, J., Azoitei, M., Glekas, G., *Danuser, G., *Sondek, J., and *Hahn, K.M. Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis.. Nature Chem. Biol., 16(8): 826-833, 2020. PMC7388658. Online article | Free PMC article
Teets, F.D., Watanabe, .T, Hahn, K.M., and Kuhlman, B. A Computational Protocol for Regulating Protein Binding Reactions with a Light-Sensitive Protein Dimer. J. Mol. Biol., 432(4): 805-814, 2020. PMC7054171. Online article | Free PMC article
O'Shaugnessy, E.C., Stone, O.J., LaFosse, P.K., Azoitei, M.L., Tsygankov, D., Heddleston, J.M., Legant, W.R., Wittchen, E.S., Burridge, K., Elston, T.C., Betzig, E., Chew, T.L., Adalsteinsson, D., and Hahn, K.M. Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor. J. Cell Biol., 218(9): 3153-3160, 2019. PMC6719445. Online article | Free PMC article
Azoitei, M.L., Noh, J., Marston, D.J., Roudot, P., Marshall, C.B., Daugird, T.A., Lisanza, S.L., Sandí, M.J., Ikura, M., Sondek, J., Rottapel, R., *Hahn, K.M., and *Danuser, G. Spatiotemporal dynamics of GEF-H1 activation controlled by microtubule- and Src-mediated pathways. J. Cell Biol., 218(9): 3077-3097, 2019. PMC6719461. Online article | Free PMC article
MacNevin, C.J., Watanabe, T., Weitzman, M., Gulyani, A., Fuehrer, S., Pinkin, N.K., Tian, X., Liu, F., Jin, J., and Hahn, K.M. Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells. J. Am. Chem. Soc. 141(18): 7275-7282., 2019. PMC6572722. Online article | Free PMC article
Woroniuk, A., Porter, A., White, G., Newman, D.T., Diamantopoulou, Z., Waring, T., Rooney, C., Strathdee, D., Marston, D.J., Hahn, K.M., Sansom, O.J., Zech, T., and Malliri, A. STEF/TIAM2-mediated Rac1 activity at the nuclear envelope regulates the perinuclear actin cap. Nature Commun., 9(1):2124, 2018. PMC5974301. Online article | Free PMC Article
*Hodgson, L., *Spiering, D., Sabouri-Ghomi, M., Dagliyan, O., DerMardirossian, C., *Danuser, G. and *Hahn, K.M. FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nature Chem. Biol., 12(10): 802-9, 2016. PMC5030135. Online article | Free PMC article
MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V. and Hahn, K.M. Ratiometric imaging using a single dye enables simultaneous visualization of Rac1 and Cdc42 activation. J. Am. Chem. Soc., 138(8): 2571-2575, 2016. PMC4825053. Online article | Free PMC article
Yumerefendi, H., Dickinson, D.J., Wang, H., Zimmerman, S., Bear, J.E., Goldstein, B., *Hahn, K. and *Kuhlman, B. Control of protein activity and cell fate specification via light-mediated nuclear translocation. PLoS One, 10(6): e0128443, 2015. PMC4471001. Online article | Free PMC article
Zawistowski, J.S., Sabouri-Ghomi, M., Danuser, G., *Hahn, K.M., and *Hodgson, L. A RhoC Biosensor Reveals Differences in the Activation Kinetics of RhoA and RhoC in Migrating Cells. PLoS One 8(11): e79877, 2013. PMC3818223. Online article | Free PMC article
Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., Plückthun, A. Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells. Chem. Biol. 20(6): 847-56, 2013. PMC4154710. Online article | Free PMC article
Jha, R.K., Wu, Y.I., Zawistowski, J., MacNevin, C., *Hahn, K.M. and *Kuhlman B. Redesign of the PAK1 auto-inhibitory domain for enhanced stability and affinity in biosensor applications. J. Mol. Biol., 413(2): 513-522, 2011. PMC3202338. Online article | Free PMC article
Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston T., and Hahn, K.M. A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem. Biol., 7: 437-444, 2011. PMC3135387. Online article. | Free PMC article
Shadpour, H., Zawistowski, J.S., Herman, A., Hahn, K.M., and Allbritton, N.L. Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors. Analytica Chimica Acta, 696: 101-107, 2011. PMC3104244. Online article | Free PMC article
Machacek, M., Hodson, L., Welch, C., Elliot, H., Pertz, O., Nalbant, P., Abell, A., Johnson, G., *Hahn, K.M. and *Danuser, G. Coordination of Rho GTPase activities during cell protrusion. Nature, 461:99-103, 2009. PMC2885353. Online article | Free PMC article
Hodgson, L., Pertz, O., and Hahn, K.M. Design and Optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol., 85:63-81, 2008. Online article | PDF
Cai, X., Lietha, D. Ceccarelli, D.F., Karginov, A.V., Rajfur, Z., Jacobson, K., Hahn, K.M., Eck, M.J., and Schaller, M.D. Spatial and temporal regulation of focal adhesion kinase activity in living cells. Mol. Cell Biol. 28:201-14, 2008. PMC2223290. Online article | Free PMC article
Hodgson, L., Chan, E.W.L., Hahn, K.M., and Yousaf, M.N. Combining Surface Chemistry with a FRET-Based Biosensor to Study the Dynamics of RhoA GTPase Activation in Cells on Patterned Substrates. J. Am. Chem. Soc. 129:9264 - 9265, 2007. Online article | PDF
Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF`
Pertz, O., Hodgson, L., Klemke, R.R., and Hahn, K.M. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature, 440(7087):1069-1072, 2006. Online article | PDF
Weiss, T.S., Chamberlain, C.E., Takeda, T., Lin, P., Hahn, K.M. and Farquhar, M.G. Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins. Proc. Natl. Acad. Sci. U.S.A., 98: 14961-14966, 2001. PMC64966. Online article | PMC Free article
Kraynov, V. S., Chamberlain, C. E., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., and K.M. Hahn. Localized Rac activation dynamics visualized in living cells. Science, 290:333-337, 2000. Online article | PDF
Chamberlain, C.E., Kraynov, V. and Hahn, K.M. Imaging spatiotemporal dynamics of Rac activation in vivo with FLARE. Methods Enzymol., 325: 389-400, 2000. Online article | PDF
Bark, S. J., and K.M. Hahn. Fluorescent indicators of peptide cleavage inside living cells. Methods, 20: 429-435, 2000. Online article | PDF
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