hahn lab

biosensors

Back to main pubs page

Hodgson, L., Spiering, D., Sabouri-Ghomi, M., DerMardirossian, C., Danuser, G., and Hahn, K.M. FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nature Chem. Biol., 12(10):802-9, 2016. PMC5030135.

MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V., Hahn, K.M. Ratiometric Imaging Using a Single Dye Enables Simultaneous Visualization of Rac1 and Cdc42 Activation. J. Am. Chem. Soc., 38(8): 2571-5, 2016. PMC4825053.

O'Shaughnessy, E.C., Yi, J.J., and Hahn, K.M. Biosensors of GTPase proteins for use in living cells and animals, in Optical Probes in Biology, Zhang, J. and Schultz, C., editors, Taylor & Francis Books, Inc., 2015, Chp. 5, pp. 137-166.

Slattery, S.D. and Hahn, K.M. A high content assay for biosensor validation and for examining stimuli that affect biosensor activity.  Current Protocols in Cell Biology, 2014, 65:14.15.1–14.15.31. PMC4324324.

Khalil, B.D., Hanna, S., Saykali, B.A., El-Sitt, S., Nasrallah, A., Marston, D., El-Sabban, M., Hahn, K.M., Symons, M., and El-Sibai, M. The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility. Exp. Cell Res. 321(2):109-122, 2014. PMC4297755

Zawistowski, J.S., Sabouri-Ghomi, M., Danuser, G., Hahn, K.M.*, and Hodgson, L. A.* RhoC Biosensor Reveals Differences in the Activation Kinetics of RhoA and RhoC in Migrating Cells. PLoS One 8(11): e79877, 2013. PMC3818223

Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., Plückthun, A.  Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells.  Chem. Biol. 20(6): 847-56, 2013. PMC415710

MacNevin, C.J., Gremyachinskiy, D., Hsu, C.W., Li L., Rougie, M., Davis, T.T., Hahn, K.M. Environment-sensing merocyanine dyes for live cell imaging applications. Bioconjug. Chem. 24(2):215-23, 2013. PMC3667669

Hinde, E., Digman, M.A., Hahn, K.M., and Gratton, E. Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM. Proc Natl Acad Sci U.S.A. 110(1): 135-40, 2013. PMC3538204

Vilela M, Halidi N, Besson S, Elliott H, Hahn K, Tytell J, Danuser G. Fluctuation analysis of activity biosensor images for the study of information flow in signaling pathways. Methods Enzymol.  519:253-76, 2013.  PMC4174660

Collins, C., Guilluy, C., Welch, C., O’Brien, E.T., Hahn, K.M., Superfine, R., Burridge,K., and Tzima, E.  Localized tensional forces on PECAM-1 elicit a global mechanotransduction response via the integrin-RhoA pathway. Curr. Biol. 22(22): 2087-94, 2012. PMC3681294

Hinde, E., Digman, M.A., Welch, C., Hahn, K.M., and Gratton, E. Biosensor FRET detection by the phasor approach to Fluorescence Lifetime Imaging Microscopy (FLIM). Microsc Res Tech 75(3): 271-81, 2012.  PMC3523109

Helassa, N., Garnett, J.P., Farrant, M., Khan, F., Pickup, J.C., Hahn, K.M., MacNevin, C.J., Tarran, R., and Baines, D.L. A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochem. J. 464(2):213-20, 2014. PMC4357280

Welch, C.M., Elliott, H., Danuser, G., and Hahn, K.M. Imaging the coordination of multiple signaling activities in living cells. Nature Reviews Molecular Cell Biology. 12: 749-756, 2011. PMC3773929

Jha, R.K., Wu, Y.I., Zawistowski, J., MacNevin, C., Hahn, K.M.*, and Kuhlman B.* Redesign of the PAK1 Auto-Inhibitory Domain for enhanced stability and affinity in biosensor applications.   J. Mol. Biol. 413(2): 513-22, 2011. PMC3202338

Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston T., and Hahn, K.M.  A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem Bio 7:437-444, 2011.  PMC3135387.

Shadpour, H., Zawistowski, J.S., Herman, A., Hahn, K.M., and Allbritton, N.L.  Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors. Analytica Chimica Acta, 696: 101-107, 2011. PMC3104244

Hodgson, L,. Shen, F., and Hahn, K. Current Protocols in Cell Biology. 46 ed. Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, Yamada KM, editors. Hoboken, NJ: Wiley & Sons, Inc.; 2010. Chp.14.11, Biosensors for characterizing the dynamics of Rho family GTPases in living cells; p. 14.11.1-14.11.26.  PMC2998069

Koivusalo, M., Welch, C., Hayashi, H., Scott, C.C., Kim, M., Alexander, T., Touret, N., Hahn, K.M., and Grinstein, S. Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signalling. J. Cell Biol., 188: 547-563, 2010. PMC2828922

Machacek, M., Hodson, L., Welch, C., Elliot, H., Pertz, O., Nalbant, P., Abell, A., Johnson, G., Hahn, K.M.* and Danuser, G.* Coordination of Rho GTPase activities during cell protrusion. Nature, 461: 99-103, 2009. PMC2885353.

Su, L., Pertz, O., Mikawa, M., Hahn, K.M., and Parsons, S.J. p190RhoGAP negatively regulates Rho activity at the cleavage furrow of mitotic cells. Exper.Cell Res., 315: 1347-59, 2009. PMC2731427

Manukyan, M., Nalbant, P., Hahn, K.M., and Knaus, U.G. RhoA GTPase activation by TLR ligands: connecting via Src to NF-kB. J. Immunol. 182(6):3522-3529, 2009. PMC2684960

El-Sibai, M., Pertz, O., Pang, H., Yip, S.C., Lorenz, M., Symons, M., Condeelis, J.S., Hahn, K.M, and Backer, J.M. RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells. Exp. Cell Res. 314:1540-52, 2008. PMC2677995

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476

Hodgson, L., Pertz, O., and Hahn, K.M. Design and Optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol, 85:63-81, 2008.

Cai, X., Lietha, D. Ceccarelli, D.F., Karginov, A.V., Rajfur, Z., Jacobson, K., Hahn, K.M., Eck, M.J., and Schaller, M.D. Spatial and temporal regulation of focal adhesion kinase activity in living cells. Mol. Cell Biol. 28:201-14, 2008. PMC2223290

Hodgson, L., Pertz, O., and Hahn, K.M. Design and optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol. 85:63-81, 2008, Epub date:July 2007.

Frantz, C.,  Karydis, A., Nalbant, P., Hahn, K. M., and Barber, D. L.  Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells.  J. Cell Biol. 179: 403-410, 2007. PMC2064788

El-Sibai, M., Nalbant, P., Sarmiento, C., Macaluso, F., Cammer, M, Condeelis, J.S., Hahn, K.M. and Backer, J.M. Cdc42 is Required for EGF-Stimulated Protrusion and Motility in Carcinoma Cells. J. Cell Science, 120(Pt 19):3465-74, 2007.

Hodgson, L., Chan, E.W.L., Hahn, K.M., and Yousaf, M.N. Combining Surface Chemistry with a FRET-Based Biosensor to Study the Dynamics of RhoA GTPase Activation in Cells on Patterned Substrates.  J. Am. Chem. Soc. 129:9264 - 9265, 2007.

Birkenfeld, J., Nalbant, P., Bohl, B.P., Pertz, O.P., Hahn, K.M., and Bokoch, G.M. GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases. Developmental Cell. 12:699-712, 2007. PMC1965589

Van Keymeulen, A., Wong, K., Knight, Z.A., Govaerts, C., Hahn, K.M., Shokat, K.M., and Bourne, H.R. To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front. J. Cell Biol., 174:437-445, 2006. PMC2064239

Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006.

Nakamura, K., Uhilk, M.T., Johnson, N.L., Hahn, K.M., and Johnson, G.L. PB1 domain-dependent signaling complex is required for extracellular signal-regulated kinase 5 activation. Mol. Cell Biol., 26:2065-2079, 2006. PMC1430298

Wong, K., Pertz, O., Hahn, K., and Bourne, H. Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism. Proc Natl Acad Sci U S A., 103:3639-44, 2006. PMC1450135

Pertz, O., L. Hodgson, R. R. Klemke, and K.M. Hahn. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature,  440(7087):1069-1072, 2006.

Pertz, O. and K.M. Hahn.  Designing biosensors for Rho family proteins – deciphering the dynamics of Rho family GTPase activation in living cells. J. Cell Sci. 117(8): 1313-1318, 2004.

Nalbant, P., L. Hodgson, V. Kraynov, A. Toutchkine, K.M. Hahn. Activation of Endogenous Cdc42 Visualized in Living Cells. Science, 305: 1615-1619, 2004.

Tan, X., P. Nalbant, A. Toutchkine, D. Hu, E. R. Vorpagel, K.M. Hahn, and H.P. Lu. Single Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System. J. Phys. Chem. B 108: 737-744, 2004.

Gaits,F. and Hahn, K.M. Shedding Light on Cell Signaling: Interpretation of FRET Biosensors. Sci. STKE 2003, p3, 2003.

Toutchkine, A. and Hahn, K.M. Live cell fluorescent biosensors for activated signaling proteins. Curr. Op Cell Biol.  14:167-172, 2002.

Gardiner, E.M., N. Pestonjamasp, B.P. Bohl, C. Chamberlain, K.M. Hahn, and G.M. Bokoch. Spatial and temporal analysis of Rac activation during live neutrophil chemotaxis:  Roles in leading edge extension and tail retraction. Current Biology, 12:2029-2034, 2002.

Katsumi, A., J. Milanini, W.B. Kiosses, M.A.del Pozo, R. Kaunas, S. Chien, K.M. Hahn, and M.A. Schwartz. Effects of cell tension on membrane protrusion through regulation of the GTPase Rac. J. Cell Biol., 158:1-13, 2002. PMC2173027.

Del Pozo, M.A., W.B. Kiosses, N.B. Alderson, N. Meller, K.M. Hahn, and A. Schwartz. Integrin-regulated membrane translocation of GTP-Rac induces localized interaction with effectors due to dissociation of RhoGDI. Nature Cell Biology, 4: 232-239, 2002.

Weiss, T. S., C. E. Chamberlain, T. Takeda, P. Lin, K. M Hahn and M. G. Farquhar. Live-Cell fluorescence resonance energy transfer (FRET) shows Interaction between Gai3 on golgi membranes and the cytosolic pool of calnuc. Proc. Natl. Acad. Sci., USA. 98:14961-14966, 2001. PMC64966

Chamberlain, C. E., and Hahn, K.M. Watching proteins in the wild – fluorescence methods to study protein dynamics in living cells. Traffic, 1: 755-762, 2000.

Kraynov, V. S., Chamberlain, C. E., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., and K. M. Hahn. Localized Rac activation dynamics visualized in living cells. Science, 290: 333-337, 2000.

Chamberlain, C. E., V. Kraynov and K. M. Hahn. Imaging Spatiotemporal Dynamics of Rac Activation in vivo with FLAIR. Methods in Enzymology, 325:389-400, 2000.

Bark, S. J., S. Schmid, and K.M. Hahn. A highly efficient method for site specific modification of peptides after chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000.

Hahn, K.M., J. Kolega, J. Montibeller, R. DeBiasio, P. Post, J. Myers and D.L. Taylor. Fluorescent analogs: Optical biosensors of the chemical and molecular dynamics of macromolecules in living cells. In Fluorescent and Luminescent Probes for Biological Activity, 2nd Ed. W.T. Mason, ed., Academic Press, London, 1999

Giuliano, K.A., P.L. Post, and Hahn, K.M. and D.L. Taylor. Fluorescent Protein Biosensors: Measurement of Molecular Dynamics in Living Cells. In The Cytoskeleton. Spudich, ed. Annual Reviews Inc., 107-136, 1996.

Giuliano, K., P.L. Post, Hahn, K.M. and D.L. Taylor.  Fluorescent protein biosensors: measurement of molecular dynamics in living cells.  Ann. Rev. Biophys. Biomol.Struct., 24:405-434, 1995.

Hahn, K.M., R. DeBiasio and D.L. Taylor.  Patterns of elevated free calcium and calmodulin activation in living cells.  Nature, 359: 736-738, 1992.

Hahn, K.M., A.S. Waggoner and D.L. Taylor.  A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin- binding fluorophore.  J. Biol. Chem., 265: 20335-20345, 1990.

Back to top
Back to main pubs page

 

Home | People | Alumni | Publications | Tools & Reagents | Cell Cinema | Links | Contacts | Join Us | Webmaster | ©2011 UNC Department of Pharmacology