hahn lab

biosensors & tools for protein control

In parentheses after each protein we list the designs or methods that were applied to it. These are linked to brief descriptions of each method. The methods are also described on the "get tools" page.

Biosensors
AR = affinity reagent, a protein or protein fragment that binds selectively to a given state of the target molecule.

Coming soon: GEFs, RhoG, Rap1, single chain biosensor versions, analogs for multiplexing

Activate with light

Coming soon: RhoG, Cofilin, GEF-H1, α-TAT

Inhibit with light

Engineeered activation by small molecules

Currently this approach has been implemented for protein activation by rapamycin. You can use rapamycin, or rapamycin analogs that do not affect the mTor pathway.  You can simply add rapamycin to the extracellular medium, for maximal activation in 30-60 seconds for most proteins. For more rapid activation and more precise control o flocalizations, we have published a photo-activatable rapamycin analog (J. Am. Chem. Soc., 133: 420-423, 2011). PMC3133816). With this  you first incubate with the rapamycin analog to load your cells, then irradiate when activation is desired.

1) Simply activate

Coming soon - uniRapR Lyn, uniRapR versions

2) The activated molecule interacts specifically with only one of its downstream targets

3) Rapamycin-induced targeting to the cell membrane

 

 

©2018 UNC Department of Pharmacology