hahn lab

optogenetics

Photocontrol of Rac1, Cdc42 and dominant negative Rac1
(PA-Rac1, PA-Cdc42, dominant negative PA-Rac1)

Protein analogs activated by light. Note that we had to place a point mutation in the effector binding region of Cdc42.

 

Methods Article

 

Example Applications


Photocontrol of endogenous PKA and MLCK
(PA-MKI, PA-PKI)

Light controlled peptides that inhibit endogenous protein activity.


Photocontrol of Diaphonous-related formins
(PA-DAD)

A 'caged' DAD domain of hDia1 is used to activate endogenous Diaphanous-related formins.


Photocontrol of dimerization, and of a peptide that potentially inhibits vinculin-talin interaction and activates vinculin

(LOV-ipaA, LOV-SsrA)

'Caged' versions of the ipaA and SsrA peptides bind their targets with enhanced affinity in the light. These switches were used for photo-activatable gene transcription in yeast. Limited attempts to alter adhesion dynamics using caged LOV-ipaA in mammalian cells were unsuccessful.


Photocontrol of nuclear translocation
(LANS)

Control the exposure of nuclear localization and nuclear export sequences, thus regulating nuclear localization with light.

 

back to tools & reagents page

Home | People | Alumni | Publications | Tools & Reagents | Cell Cinema | Links | Contacts | Join Us | Webmaster | ©2011 UNC Department of Pharmacology