hahn lab

protein analog biosensors

FRET

 

Binder-tag

 

Dye-labeled proteins

Hahn, K.M., A.S. Waggoner and D.L. Taylor.  A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin- binding fluorophore.  J. Biol. Chem., 265: 20335-20345, 1990. Online article | PDF

Hahn, K.M., R. DeBiasio and D.L. Taylor.  Patterns of elevated free calcium and calmodulin activation in living cells. Nature, 359:736-738, 1992. Online article | PDF

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476. Online article | Free PMC article


Detailed methods

Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF
*Note: this article is focused on dye-based biosensors, but includes a method to correct for bleaching of any fluorophore.

Hodgson, L., Pertz, O., and Hahn, K.M. Design and Optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol, 85:63-81, 2008. [Link downloads PDF] Online article
*Note: this older methods article is for genetically encoded biosensors but contains useful steps for analysis of ratiometric protein analogsdes.

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