hahn lab

dyes and site-specific labeling for biosensors

Dyes

Toutchkine, A., Nalbant, P., and Hahn, K.M. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility.  Bioconjugate Chem., 3:386-389, 2002. Onine article | PDF

Toutchkine, A., V. Kraynov, and K.M. Hahn. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living Cells. J. Amer. Chem. Soc., 125:4132-4145, 2003. Online article | PDF

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Simple one-pot preparation of water-soluble, cysteine-reactive cyanine and merocyanine dyes for biological imaging. Bioconjugate Chem. 18:1344-1348, 2007. PMC3694264. Online article | Free PMC article

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Merocyanine dyes with improved photostability. Org. Lett., 9: 2775-2777, 2007. Online article | PDF

MacNevin, C.J., Gremyachinskiy, D., Hsu, C.W., Li L., Rougie, M., Davis, T.T., Hahn, K.M. Environment-sensing merocyanine dyes for live cell imaging applications. Bioconjug. Chem. 24(2):215-23, 2013. PMC3667669. Online article | Free PMC article

MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V. and Hahn, K.M. Ratiometric imaging using a single dye enables simultaneous visualization of Rac1 and Cdc42 activation. J. Am. Chem. Soc., 138(8): 2571-2575, 2016. PMC4825053. Online article | Free PMC article


Physical chemistry of dyes

Toutchkine, A., Han, W.G., Ullmann, M., Liu, T., Bashford, D., Noodleman, L., and Hahn, K.M. Experimental and DFT studies: novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes. J. Phys. Chem. A., 111:10849-60, 2007. PMC3742023. Online article | Free PMC article

Liu, T., Han, W., Himo, F., Ullmann, G.M., Bashford, D., Toutchkine,A., Hahn, K.M., and Noodleman, L. Density Functional Vertical Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A 108: 3545-3555, 2004. [Link downloads PDF]

Han, W. G., Liu, T., Himo, F., Toutchkine, A., Bashford, D., Hahn, K.M., and Noodleman, L. A Theoretical Study of the UV Absorption and Emission Solvatochromic Properties of Solvent Sensitive Dyes. ChemPhysChem, 4:1084-1094, 2003. Online article | PDF


Site-specific labeling

Cornish, V. W., K. M. Hahn, and P. G. Schultz. Site-specific protein modification using a ketone handle. J. Am. Chem. Soc., 118: 8150-8151, 1996. PMC2121065 [Link downloads PDF] Online article

Bark, S. J., S. Schmid, and K.M. Hahn. A highly efficient method for site specific modification of peptides after chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000. [Link downloads PDF] | Online article


Labeling in living cells

 

©2018 UNC Department of Pharmacology