hahn lab

small molecules to visualize and control proteins

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Dagliyan, O., Tarnawski, M., Chu, P-H., Shirvanyants, D., Schlichting, I., Dokholyan, N.V., and Hahn, K.M. Engineering extrinsic disorder to control protein activity in living cells.  Science, 354(6318): 1441-1444, 2016.

MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T.,Nguyen, D.V., and Hahn, K.M. Ratiometric Imaging Using a Single Dye Enables Simultaneous Visualization of Rac1 and Cdc42 Activation. J. Am. Chem. Soc., 138(8):2571-5, 2016. PMCID: PMC4825053

Helassa, N., Garnett, J.P., Farrant, M., Khan, F., Pickup, J.C., Hahn, K.M., MacNevin, C.J., Tarran, R., and Baines, D.L. A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochem. J. 464(2):213-20, 2014. PMC4357280

Karginov, A., Tsygankov, D., Berginski, M., Chu, P-H., Trudeau, E., Yi, J.J., Gomez, Shawn, Elston, T.C. and Hahn, K.M. Dissecting motility signaling through activation of specific Src-effector complexes. Nat. Chem. Bio. 10(4):286-90, 2014.  PMC4064790

Konze, K.D., Ma, A., Li, F., Barsyte-Lovejoy, D., Parton, T., Macnevin, C.J., Liu, F., Gao, C., Huang, X.P., Kuznetsova, E., Rougie, M., Jiang, A., Pattenden, S.G., Norris, J.L., James, L.I., Roth, B.L., Brown, P.J., Frye, S.V., Arrowsmith, C.H., Hahn, K.M., Wang, G.G., Vedadi, M., Jin, J. An Orally Bioavailable Chemical Probe of the Lysine Methyltransferases EZH2 and EZH1. ACS Chem. Biol. 8 (6): 1324–1334, 2013. PMC3773059

Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., Plückthun, A.  Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells.  Chem. Biol. 20(6): 847-56, 2013. PMC415710

Dagliyan, O., Shirvanyants, D., Karginov, A.V., Ding, F., Fee, L., Chandrasekaran, S.N., Freisinger, C.M., Smolen, G.A., Huttenlocher, A., Hahn, K.M.*, Dokholyan, N.V.* Rational design of a ligand-controlled protein conformational switch. Proc. Natl. Acad. Sci. U. S. A. 110(17): 6800-4, 2013. PMC3637791

MacNevin, C.J., Gremyachinskiy, D., Hsu, C.W., Li L., Rougie, M., Davis, T.T., Hahn, K.M. Environment-sensing merocyanine dyes for live cell imaging applications. Bioconjug. Chem. 24(2):215-23, 2013. PMC3667669

Karginov, A.V., and Hahn, K.M. Allosteric activation of kinases: design and application of RapR kinases. Curr. Protoc. Cell Biol., Chapter 14:Unit 14.13, 2011. PMC3269071

Karginov, A.V., Zou, Y.,  Shirvanyants, D., Kota, P., Dokholyan, N.V., Young, D.D., Hahn, K.M.*, and Deiters, A.*  Light Regulation of Protein Dimerization and Kinase Activity in Living Cells Using Photocaged Rapamycin and Engineered FKBP. J. Am. Chem. Soc., 133: 420-423, 2011. PMC3133816

Karginov, A., Ding, F., Kota, P., Dokholyan, N.V., and Hahn, K.M. Engineered allosteric activation of kinases in living cells. Nature Biotech.,28(7): 743-7, 2010.  PMC2902629   

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Merocyanine dyes with improved photostability. Org. Lett. 9: 2775-2777, 2007.

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Simple one-pot preparation of water-soluble, cysteine-reactive cyanine and merocyanine dyes for biological imaging. Bioconjugate Chem. 18:1344-1348, 2007. PMC3694264

Liu, T., Han, W., Himo, F., Ullmann, G.M., Bashford, D., Toutchkine,A., Hahn, K.M., and Noodleman, L. Density Functional Vertical Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A 108: 3545-3555, 2004.

Han, W. G., Liu, T., Himo, F., Toutchkine, A., Bashford, D., Hahn, K.M., and Noodleman, L. A Theoretical Study of the UV Absorption and Emission Solvatochromic Properties of Solvent Sensitive Dyes. ChemPhysChem, 4:1084-1094, 2003.

Toutchkine, A.,V. Kraynov, and K. M. Hahn. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living Cells, J. Amer. Chem. Soc., 125:4132-4145, 2003.

Liu, T., W. Han, F. Himo, G. M. Ullmann, D. Bashford, A. Toutchkine, K. M. Hahn, L. Noodleman. Density Functional Vertical Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A 108: 3545-3555, 2004.

Toutchkine, A., Nalbant, P., and Hahn, K.M. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility.  Bioconjugate Chem., 3:386-389, 2002.

Toutchkine, A., P. Nalbant and K.M. Hahn. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility.  Bioconjugate Chem., 3:386-389, 2002.

Bark, S. J., S. Schmid, and K.M. Hahn. A highly efficient method for site specific modification of peptides after chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000.

Bark, S. J., and K.M. Hahn. Fluorescent indicators of peptide cleavage inside living cells. Methods, 20: 429-435, 2000.

Cornish, V. W., K. M. Hahn, and P. G. Schultz. Site-specific protein modification using a ketone handle. J. Am. Chem. Soc., 118: 8150-8151, 1996. PMC2121065

Knauf, P.A., F.Y. Law, and K.M. Hahn.  An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange.  Am. J. Physiol. (Cell Physiol.), C 1073-C1077, 1995.

Southwick, P., K.M. Hahn, J. Chao, P. Parry, A.S. Wagman, M. Wagner and A.S. Waggoner.  A long wavelength biolabeling reagent based on the oxonol flurophore.  J. Fluorescence, 5(2): 231-235, 1995.

Hahn, K.M., P.A. Conrad, J. Chao, D.L. Taylor and A.S. Waggoner.  A photocrosslinking, fluorescent indicator of mitochondrial membrane potential.  J. Histochem. Cytochem., 41: 631-634, 1993.

Hahn, K.M., R. DeBiasio and D.L. Taylor.  Patterns of elevated free calcium and calmodulin activation in living cells.  Nature, 359: 736-738, 1992.

Hahn, K.M., S.B. Hastie and R.J. Sundberg.  Synthesis and evaluation of 2 diazo 3,3,3 trifluoropropanoyl derivatives of colchicine and podophyllotoxin as photoaffinity labels: reactivity, photochemistry, and tubulin binding.  Photochem. Photobiol., 55: 17-27, 1992.

Hahn, K.M. and P.L. Southwick.  A stable diazo photoaffinity label with increased absorptivity and effective photoactivation beyond 300 nm. Anal. Biochem., 196: 271-278, 1991.

Hahn, K.M., W.G. Humphries, A.M. Helms, S.B. Hastie and T.L. Macdonald.  Structural requirements for the binding of colchicine analogs to tubulin: the role of the C-10 substituent.  Biomed. Chem. Lett., 1: 471-476, 1991.

Hahn, K.M., A.S. Waggoner and D.L. Taylor.  A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin- binding fluorophore.  J. Biol. Chem., 265: 20335-20345, 1990.

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