hahn lab

dyes; site-specific protein modification; chemical biology

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Ye, F., Yang, C., Kim, J., MacNevin, C.J., Hahn, K.M., Park, D., Ginsberg, M.H., and Kim, C. Epigallocatechin Gallate Has Pleiotropic Effects on Transmembrane Signaling by Altering the Embedding of Transmembrane Domains. J. Biol. Chem., 292(24): 2858-2864, 2017. PMC5473239 Online article | Free PMC article

MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V. and Hahn, K.M. Ratiometric imaging using a single dye enables simultaneous visualization of Rac1 and Cdc42 activation. J. Am. Chem. Soc., 138(8): 2571-2575, 2016. PMC4825053. Online article | Free PMC article

Helassa, N., Garnett, J.P., Farrant, M., Khan, F., Pickup, J.C., Hahn, K.M., MacNevin, C.J., Tarran, R., and Baines, D.L. A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochem. J. 464(2):213-20, 2014. PMC4357280. Online article | Free PMC article

Konze, K.D., Ma, A., Li, F., Barsyte-Lovejoy, D., Parton, T., Macnevin, C.J., Liu, F., Gao, C., Huang, X.P., Kuznetsova, E., Rougie, M., Jiang, A., Pattenden, S.G., Norris, J.L., James, L.I., Roth, B.L., Brown, P.J., Frye, S.V., Arrowsmith, C.H., Hahn, K.M., Wang, G.G., Vedadi, M. and Jin, J. An orally bioavailable chemical probe of the lysine methyltransferases EZH2 and EZH1. ACS Chem. Biol., 8(6): 1324–1334, 2013. PMC3773059. Online article | Free PMC article

Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., Plückthun, A.  Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells.  Chem. Biol. 20(6): 847-56, 2013. PMC415710. Online article | Free PMC article

MacNevin, C.J., Gremyachinskiy, D., Hsu, C.W., Li L., Rougie, M., Davis, T.T., Hahn, K.M. Environment-sensing merocyanine dyes for live cell imaging applications. Bioconjug. Chem. 24(2):215-23, 2013. PMC3667669. Online article | Free PMC article

Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston T. and Hahn, K.M.  A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem. Biol., 7: 437-444, 2011. PMC3135387. Online article | Free PMC article

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476. Online article | Free PMC article

Toutchkine, A., Han, W.G., Ullmann, M., Liu, T., Bashford, D., Noodleman, L., and Hahn, K.M. Experimental and DFT studies: novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes. J. Phys. Chem. A., 111:10849-60, 2007. PMC3742023. Online article | Free PMC article

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Merocyanine dyes with improved photostability. Org. Lett., 9: 2775-2777, 2007. Online article | PDF

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Simple one-pot preparation of water-soluble, cysteine-reactive cyanine and merocyanine dyes for biological imaging. Bioconjugate Chem. 18:1344-1348, 2007. PMC3694264. Online article | Free PMC article

Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF

Liu, T., Han, W., Himo, F., Ullmann, G.M., Bashford, D., Toutchkine,A., Hahn, K.M., and Noodleman, L. Density Functional Vertical Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A 108: 3545-3555, 2004. [Link downloads PDF]

Tan, X., Nalbant, P., Toutchkine, A., Hu, D., Vorpagel, E.R., Hahn, K.M. and Lu, H.P. Single molecule study of protein-protein interaction dynamics in a cell signaling system. J. Phys. Chem. B, 108: 737-744, 2004. [Link downloads PDF]

Han, W. G., Liu, T., Himo, F., Toutchkine, A., Bashford, D., Hahn, K.M., and Noodleman, L. A Theoretical Study of the UV Absorption and Emission Solvatochromic Properties of Solvent Sensitive Dyes. ChemPhysChem, 4:1084-1094, 2003. Online article | PDF

Toutchkine, A., V. Kraynov, and K.M. Hahn. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living Cells. J. Amer. Chem. Soc., 125:4132-4145, 2003. Online article | PDF

Toutchkine, A., Nalbant, P., and Hahn, K.M. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility.  Bioconjugate Chem., 3:386-389, 2002. Onine article | PDF

Bark, S. J., S. Schmid, and K.M. Hahn. A highly efficient method for site specific modification of peptides after chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000. [Link downloads PDF] | Online article

Bark, S. J., and K.M. Hahn. Fluorescent indicators of peptide cleavage inside living cells. Methods, 20: 429-435, 2000. Online article | PDF

Cornish, V. W., K. M. Hahn, and P. G. Schultz. Site-specific protein modification using a ketone handle. J. Am. Chem. Soc., 118: 8150-8151, 1996. PMC2121065 [Link downloads PDF] Online article

Knauf, P.A., F.Y. Law, and K.M. Hahn.  An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange.  Am. J. Physiol. (Cell Physiol.), C 1073-C1077, 1995. Online article | PDF

Southwick, P., K.M. Hahn, J. Chao, P. Parry, A.S. Wagman, M. Wagner and A.S. Waggoner.  A long wavelength biolabeling reagent based on the oxonol flurophore.  J. Fluorescence, 5(2): 231-235, 1995. Online article | PDF

Hahn, K.M., P.A. Conrad, J. Chao, D.L. Taylor and A.S. Waggoner.  A photocrosslinking, fluorescent indicator of mitochondrial membrane potential.  J. Histochem. Cytochem., 41: 631-634, 1993. Online article | PDF

Hahn, K.M., Hastie, S.B. and Sundberg, R.J. Synthesis and evaluation of 2 diazo 3,3,3 trifluoropropanoyl derivatives of colchicine and podophyllotoxin as photoaffinity labels: reactivity, photochemistry, and tubulin binding. Photochem. Photobiol., 55: 17-27, 1992. Online article | PDF

Hahn, K.M. and Southwick, P.L. A stable diazo photoaffinity label with increased absorptivity and effective photoactivation beyond 300 nm. Anal. Biochem., 196: 271-278, 1991. Online article | PDF

Hahn, K.M., Humphries, W.G., Helms, A.M., Hastie, S.B. and Macdonald, T.L. Structural requirements for the binding of colchicine analogs to tubulin: the role of the C-10 substituent. Biomed. Chem. Lett., 1: 471-476, 1991.[link downloads PDF]

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