hahn lab

dyes, labeling, and biosensors based on environment-sensing dyes

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*= key reference

*MacNevin, C.J., Watanabe, T., Weitzman, M., Gulyani, A., Fuehrer, S., Pinkin, N.K., Tian, X., Liu, F., Jin, J., and Hahn, K.M. Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells. J. Am. Chem. Soc., 141(18): 7275-7282., 2019. PMC6572722. Online article | Free PMC article

Ye, F., Yang, C., Kim, J., MacNevin, C.J., Hahn, K.M., Park, D., Ginsberg, M.H., and Kim, C. Epigallocatechin Gallate Has Pleiotropic Effects on Transmembrane Signaling by Altering the Embedding of Transmembrane Domains. J. Biol. Chem., 292(24): 2858-2864, 2017. PMC5473239 Online article | Free PMC article

*MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V., and Hahn, K.M. Ratiometric imaging using a single dye enables simultaneous visualization of Rac1 and Cdc42 activation. J. Am. Chem. Soc., 138(8): 2571-2575, 2016. PMC4825053. Online article | Free PMC article

Helassa, N., Garnett, J.P., Farrant, M., Khan, F., Pickup, J.C., Hahn, K.M., MacNevin, C.J., Tarran, R., and Baines, D.L. A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochem. J., 464(2):213-20, 2014. PMC4357280. Online article | Free PMC article

Konze, K.D., Ma, A., Li, F., Barsyte-Lovejoy, D., Parton, T., MacNevin, C.J., Liu, F., Gao, C., Huang, X.P., Kuznetsova, E., Rougie, M., Jiang, A., Pattenden, S.G., Norris, J.L., James, L.I., Roth, B.L., Brown, P.J., Frye, S.V., Arrowsmith, C.H., Hahn, K.M., Wang, G.G., Vedadi, M. and Jin, J. An orally bioavailable chemical probe of the lysine methyltransferases EZH2 and EZH1. ACS Chem. Biol., 8(6): 1324–1334, 2013. PMC3773059. Online article | Free PMC article

Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., and Plückthun, A.  Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells.  Chem. Biol., 20(6): 847-56, 2013. PMC4154710. Online article | Free PMC article

*MacNevin, C.J., Gremyachinskiy, D., Hsu, C.W., Li L., Rougie, M., Davis, T.T., Hahn, K.M. Environment-sensing merocyanine dyes for live cell imaging applications. Bioconjug. Chem., 24(2):215-23, 2013. PMC3667669. Online article | Free PMC article

Jha, R.K., Wu, Y.I., Zawistowski, J., MacNevin, C., *Hahn, K.M. and *Kuhlman B. Redesign of the PAK1 auto-inhibitory domain for enhanced stability and affinity in biosensor applications. J. Mol. Biol., 413(2): 513-522, 2011. PMC3202338. Online article | Free PMC article

*Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston, T. and Hahn, K.M.  A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem. Biol., 7: 437-444, 2011. PMC3135387. Online article | Free PMC article

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476. Online article | Free PMC article

Toutchkine, A., Han, W.G., Ullmann, M., Liu, T., Bashford, D., Noodleman, L., and Hahn, K.M. Experimental and DFT studies: novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes. J. Phys. Chem. A, 111:10849-60, 2007. PMC3742023. Online article | Free PMC article

*Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Merocyanine dyes with improved photostability. Org. Lett., 9: 2775-2777, 2007. Online article | PDF

Toutchkine, A., Dan-Vinh Nguyen, D., and Hahn, K.M. Simple one-pot preparation of water-soluble, cysteine-reactive cyanine and merocyanine dyes for biological imaging. Bioconjugate Chem., 18:1344-1348, 2007. PMC3694264. Online article | Free PMC article

Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF

*Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A., and Hahn, K.M. Activation of endogenous Cdc42 visualized in living cells. Science, 305: 1615-1619, 2004. Onilne article | PDF

Liu, T., Han, W., Himo, F., Ullmann, G.M., Bashford, D., Toutchkine,A., Hahn, K.M., and Noodleman, L. Density Functional Vertical Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A, 108: 3545-3555, 2004. online article | PDF

Tan, X., Nalbant, P., Toutchkine, A., Hu, D., Vorpagel, E.R., Hahn, K.M. and Lu, H.P. Single molecule study of protein-protein interaction dynamics in a cell signaling system. J. Phys. Chem. B, 108: 737-744, 2004. online article | PDF

Han, W. G., Liu, T., Himo, F., Toutchkine, A., Bashford, D., Hahn, K.M., and Noodleman, L. A Theoretical Study of the UV Absorption and Emission Solvatochromic Properties of Solvent Sensitive Dyes. ChemPhysChem, 4:1084-1094, 2003. Online article | PDF

*Toutchkine, A., Kraynov, V., and Hahn, K.M. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living Cells. J. Amer. Chem. Soc., 125:4132-4145, 2003. Online article | PDF

Toutchkine, A., Nalbant, P., and Hahn, K.M. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility.  Bioconjugate Chem., 3:386-389, 2002. Onine article | PDF

*Bark, S. J., Schmid, S., and Hahn, K.M. A highly efficient method for site specific modification of peptides after chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000. Online article | PDF

Bark, S. J., and Hahn, K.M. Fluorescent indicators of peptide cleavage inside living cells. Methods, 20: 429-435, 2000. Online article | PDF

*Cornish, V. W., Hahn, K.M., and Schultz. P.G. Site-specific protein modification using a ketone handle. J. Am. Chem. Soc., 118: 8150-8151, 1996. Online article | PDF

Knauf, P.A., Law, F.Y., and Hahn, K.M.  An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchangeAm. J. Physiol. (Cell Physiol.), C1073-C1077, 1995. Online article | PDF

Southwick, P., Hahn, K.M., Chao, J., Parry, P., Wagman, A.S., Wagner, M, and Waggoner, A.S.  A long wavelength biolabeling reagent based on the oxonol flurophore.  J. Fluorescence, 5(2): 231-235, 1995. Online article | PDF

Hahn, K.M., Conrad, P.A., Chao, J., Taylor, D.L., and Waggoner, A.S.  A photocross-linking, fluorescent indicator of mitochondrial membrane potential.  J. Histochem. Cytochem., 41: 631-634, 1993. Online article | PDF

*Hahn, K.M., DeBiasio, R. and Taylor, D.L. Patterns of elevated free calcium and calmodulin activation in living cells.  Nature, 359: 736-738, 1992. Online article | PDF

Hahn, K.M., Hastie, S.B. and Sundberg, R.J. Synthesis and evaluation of 2 diazo 3,3,3 trifluoropropanoyl derivatives of colchicine and podophyllotoxin as photoaffinity labels: reactivity, photochemistry, and tubulin binding. Photochem. Photobiol., 55: 17-27, 1992. Online article | PDF

Hahn, K.M. and Southwick, P.L. A stable diazo photoaffinity label with increased absorptivity and effective photoactivation beyond 300 nm. Anal. Biochem., 196: 271-278, 1991. Online article | PDF

Hahn, K.M., Humphries, W.G., Helms, A.M., Hastie, S.B. and Macdonald, T.L. Structural requirements for the binding of colchicine analogs to tubulin: the role of the C-10 substituent. Biomed. Chem. Lett., 1: 471-476, 1991. PDF

Hahn, K.M., Waggoner A.S., and Taylor, D.L. A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin- binding fluorophore. J. Biol. Chem., 265: 20335-20345, 1990. Online article | PDF

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