Multiplexed optogenetic control, using Photo-activatable Vav2 (PA-Vav2) and Photo-inhibitable Rac1 (PI-Rac1) in the same cell. Upon irradiation, area and roundness changed as they did in response to PA-Vav2 alone, but edge dynamics were clearly reduced, unlike response to either PA-Vav2 alone or PI-Rac1 alone. Blue dot denotes irradiation. Cells were visualized using mVenus-PI-Rac1. by Onur Dagliyan.
Hahn Lab - copyright 2016.